Becca's B+, Blue Meanie, Purple Mystic From Spore Genetics & Blue Yeti LC Fun Run

[Scottay]

Looking nice!! Rock and roll!! Happy Monday.

 

[BeccaBuds]

Thanks @Scottay They are wolfing down the rice as I type... Day 6 at something like 140 hours in:

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And the Blue Yeti at almost 5.5 months... Hey, BY! Let's see some shrooms already!

 

[BeccaBuds]

Something to think about in the middle of a meteor shower...

 

[BeccaBuds]

Planned on posting these yesterday but got lazy after a big dinner. Day 8, around 188 hours. They took a bit longer but are ready to shake up today or tomorrow.
My order from MushroomSupplies.com, #5 of dung loving style substrate and a #5 All-In-One, arrived yesterday. Hella fast shipping! Bags should be ready to mix with substrate a week or so after shake.

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[Tokin Roll]

Something to think about in the middle of a meteor shower...

One of my favorite all time get high band.
That and Pink Floyd great listen on shrooms.

 

[BeccaBuds]

Listening to Rush's "Moving Pictures" album while performing the smash, shmush & shake of all 8 rice bags. Coulda done it 2 days ago. They should be ready to mix with substrate by next Friday, 5/17/24 to Monday, 5/20/24.

 

[BeccaBuds]

OMG, was just studying Mushroom Supplies' literature for their product and discovered I've miscalculated and inoculated twice as much grain as I need. Oh, well, now I'll have extra if I find contaminated bag(s), or more shrooms if not. If I end up with extra maybe I'll try dunking them, rolling them in substrate and set them on perlite with foil underneath. Blue Yeti brick still just idling along.
Day 11 since inoculation, one day post smash-n-smoosh:

 

[BeccaBuds]

Day 12 since inoc, around 48 hours since smash-n-smoosh:

 

[BeccaBuds]

You guessed it, another set of photos of fungus growing on rice. This time day 13 post inoc, day 3 post smash-n-smoosh and apparently completely grown through. I think these are ready to go into casing tomorrow, after only two weeks.

 

[BeccaBuds]

Here are animations of bags 1 & 5 progressing from day 10 to day 13:

 

[Schnookie]

I hope this goes better than my first attempt back in the last century, lol! Never got beyond contaminated petri dishes that time.

I was going to be ambitious and grow out all 4 phenotypes but space is limited and time is at a premium as per usual so here's the revised plan after a bit of backstory.
I have spore syringes, courtesy of @Spore Genetics, of B+, Blue Meanie Cubes, & Purple Mystic

, and from an outside source, a liquid culture syringe of Blue Yeti

. I've built myself a very nice SAB

, prepped jar lids

for liquid cultures or grain, obtained filter patches big and small, self-healing injection ports (SHIP), liquid culture pr-mix, micropore tape, rice bags, and from @Midwest Grow Kits, an All-In-One bag , casing substrate, horse manure and a pound of gypsum. Got 20 mesh stainless steel screens to make a grain prep/shroom drying trays, a magnetic stirrer and plastic coated stir bar magnets for the liquid cultures. Made 70% IPA from 99% IPA plus 29% distilled water. Dug out the pressure cooker and got a new bottom tray for it. Found micropore tape sux and must be layered 4 deep to filter, so by default Uncle Ben tek sux as well but now committed so put rtv silicone SHIPs on 5 bags

. Details ad infinitum...

This run, I'll be inoculating (4) 250mL jars of liquid culture pre-mix; 3 jars for the spores and one for the Blue Yeti culture. These will be for future grain inoculations & long term storage in the fridge. Each jar will get a 2mL shot.

I'm going to inoculate 2 rice bags with Purple Mystic spores, and the All-In-One (AIO) plus another rice bag with the Blue Yeti liquid culture. The liquid culture should populate the grain faster. The rice bags and AIO will each get 3mL shots by injecting 1mL, moving the needle in the grain injecting another milliliter, and then doing it a 3rd time. This way a larger area of grain gets inoculated. Once the mycelium has grown out onto the grain, I'll be mixing it up with manure substrate and running shoeboxes in a fruiting chamber. The Blue Yeti AIO will get 2 shoeboxes and the 3rd rice bag (also Blue Yeti) it's own shoebox. I have another Sterilite 1938 I'm making into a fruiting chamber, so I'll have room for 6 shoeboxes though this time only 5 will be run (knock on wood).

A couple days ago I wiped down the whole inside of the SAB with 70% IPA, closed the vents and plugged the arm holes. Within minutes the hygrometer read >99% RH so after an hour I opened the FAE ports. 24 hours later, RH had dropped to 80% but I could still smell some iso alcohol. Today the reading has dropped to 43% RH and I can detect only a trace of alcohol. Free air exchange (FAE) through the 0.3um filter patches

is working well.

Earlier today I mixed 44 grams liquid culture pre-mix powder with a liter of simmering distilled water to make stock and filled 250mL jars 1, 2, 3 & 4 and 200mL jar 7 (I had some extra, maybe another jar of B Yeti). Loaded the jars into the PC for sterilization at 15psi for 30 minutes. Put the top screen in over the jars and placed a foil covered packet of 5x5 paper towels and a foil covered packet of stainless steel tools (surgical scissors, #00 tweezers, forceps) to sterilize with the jars. Got the spores & culture out of the fridge, and as soon as the PC cools (tomorrow) and the syringes warm, into the SAB they go.

Tomorrow when the hole punch arrives I'll punch the FAE holes in the rice bags and apply the 4 layer micropore tapes and figure out how I'm going to get the micropore tape sterile. Next everything gets sprayed down and wiped with 70% alcohol and loaded into the SAB, and the (sterilized) arm hole plugs stuffed back in until the alcohol dissipates enough to use a lighter inside for needle sterilization. After that its inoculation time! Photo after.

@Bill284 @ExNavyInSTL @Freak Brothers @JohnnieJC3 @4me2kn0 @MotaMan420 @420 @Stoned Ape @Ramblinrose1965 @Nunyabiz @Tokin Roll @2Water @Slapalottapuss @Keffka @JoetheChro @Lady Cannafan @CaptainLucky @Mycelium Farmer @Spore Genetics @Midwest Grow Kits @seaofgreen18 @Treeibus @Pennywise @Pondwater @safeman @Melville Hobbes @Mitchell Florn @InTheShed @Krissi Carbone @Lerugged @con @Absorber @Porky82

If I missed anyone, please feel free to join in!

I just found out I can't buy spores, etc for delivery to GA. Effin. redneck state...

 

[BeccaBuds]

Hi @Schnookie I'm pretty certain Psilocybe Cubensis exists in the wild in your state, but if you have no experience with wild mushrooms it isn't safe hunting them without an experienced guide. Cubes are known to grow wild in cow pastures on or near old (composted) cow pies from Texas to the Carolinas and inland to Oak Ridge, Tn, so they can be found. You just have to find a safe way to hunt them.
There are European distributors that will ship spores pretty much anywhere. Mushly comes to mind. It's just like 25 years ago when seeds appeared on the internet- sometimes your order didn't make it to the mail box and all you can do is try again. Only now it's microscopic dust like spores instead of seeds.

 

[BeccaBuds]

Well, I'd intended to have the rice bags mixed into the casing mix yesterday and have pics up of 2 shoeboxes. Had to change plans when I got waylaid by major back pain, seemingly caused by food poisoning from a Little Seizures pizza. It's a little better today so hopefully I can get back on course tomorrow.
On a sad note, I found major contamination on the old Blue Yeti brick today, so it's time to toss it into my soil bin and shoot up the new Mushroom Supplies AIO with a full 10cc of Blue Yeti LC when I mix the rice bags.

 

[BeccaBuds]

This morning I transferred the rice to shoeboxes and mixed it with substrate. Bags 2 & 5 were bad, looked like trichoderma or penicillium, so I threw them out and mixed 3 bags per shoebox with around 2 pounds of substrate each. Here's a final set of photos of the rice bags this morning prior to transfer, and shot of the shoeboxes post-transfer. Decided to hold off inoculating the new AIO because the SAB would be too crowded. It's interesting that in both cases the contam was at the top of the rice only and wasn't visible prior to cutting the top off the bag. I could have spotted it in glass jars and set them aside instead of opening them and potentially contaminating everything.

 

[CaptainLucky]

I just found out I can't buy spores, etc for delivery to GA. Effin. redneck state...

@Spore Genetics doesn’t deliver to the state? CL

 

[Schnookie]

Nope. No one does. Illegal to own spores

 

[BeccaBuds]

mushly.com will ship them. Whether or not you get them, and the legality of it, is another story. Might take a few tries. I've lost seed purchases to postal inspectors in the past.